Olejar Books

Molecular based techniques have revolutionized our understanding of environmental microbiology. Over the last decade, the application of these techniques has improved our understanding of the composition, phylogeny, and physiology of microbial communities in the environment. However a thorough understanding of the limitations of these methods is essential to prevent researchers being led astray by their results. This book helps the reader to be aware of these limitations, and will therefore be invaluable for both the novice and the experienced molecular biologist. This volume contains a collection of protocols and descriptive narratives written by researchers working in diverse fields. Chapters contain valuable background information on the techniques, laboratory protocols, published examples and applications, and cautionary notes to help the readers avoid some of the common problems, either with the mechanics of the techniques themselves or in interpreting the results. In addition, chapters describing fundamental principles, advanced applications of selected techniques, and speculation on future methodological advances are also included. Many of the chapters focus on nucleic acid based methodologies and the poylmerase chain reaction (PCR). Methods based on the analysis of protein, and lipid, and the interaction of DNA analogues are also included. Written by internationally renowned authors, this book is aimed at all scientists working in the field of environmental microbiology, including those involved in the analysis of microbial diversity, the changes in microbial community structure, bioremediation, and the detection of specific microorganisms (eg pathogens) in the environment. An essential volume for anyone interested in